PRIMPOL (DNA-directed primase/polymerase protein)

FEATURES

FAMILY

eukaryotic-type primase small subunit family

DESCRIPTION

Also known as PRIPO_BOVIN, PRIMPOL. DNA primase and DNA polymerase required to tolerate replication-stalling lesions by bypassing them. Required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions. Shows a high capacity to tolerate DNA damage lesions such as 8oxoG and abasic sites in DNA. Provides different translesion synthesis alternatives when DNA replication is stalled: able to synthesize DNA primers downstream of lesions, such as ultraviolet (UV) lesions, R-loops and G-quadruplexes, to allow DNA replication to continue. Can also realign primers ahead of 'unreadable lesions' such as abasic sites and 6-4 photoproduct (6-4 pyrimidine-pyrimidinone), thereby skipping the lesion. Also able to incorporate nucleotides opposite DNA lesions such as 8oxoG, like a regular translesion synthesis DNA polymerase. Also required for reinitiating stalled forks after UV damage during nuclear DNA replication. Required for mitochondrial DNA (mtDNA) synthesis and replication, by reinitiating synthesis after UV damage or in the presence of chain-terminating nucleotides (By similarity). Prevents APOBEC family-mediated DNA mutagenesis by repriming downstream of abasic site to prohibit error-prone translesion synthesis (By similarity). Has non-overlapping function with POLH. In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (By similarity). Interacts with RPA1; leading to recruitment to chromatin and stimulate DNA primase activity. Interacts with SSBP1. Interacts with POLDIP2; leading to enhance DNA polymerase activity.

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EXTERNAL LINKS

PRIMPOL

DNA-directed primase/polymerase protein

MOLECULAR SYNOPSIS STRUCTURAL LIGANDABILITY

PRIMPOL

Molecular Synopsis