FEATURES

FAMILY

DESCRIPTION

Also known as ALKB_ECOLI, alkB, aidD. Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents).

EXTERNAL LINKS

uniprot
enzyme classification

alkB

Alpha-ketoglutarate-dependent dioxygenase AlkB

alkB

Molecular Synopsis